Kit Contents (US Version) Biology Kit BK01A

Kit Contents (US Version)

     The BK01A, BK01A-B, BK01A-S, and BK01A-BS Biology Kits contain all of the items listed below, except as noted. The BK01A-RK Biology Refill Kit contains chemicals only.    

  • Acetic acid, 6 M, 15 mL          
  • Agar powder, 10 g (sufficient to prepare 500 mL of agar medium for 100+ 50mm Petri dishes)           
  • Alligator clip lead, black           
  • Alligator clip lead, red           
  • Ammonia, 6 M, 15 mL         
  • Ammonium nitrate1          
  • Antibiotic powder, penicillin2          
  • Antibiotic powder, oxytetracycline           
  • Antibiotic powder, sulfamethazine           
  • Antibiotic powder, erythromycin          
  • Ascorbic acid tablets, 500 mg           
  • Barfoed's reagent, 30 mL           
  • Beaker, PP, 50 mL          
  • Beaker, PP, 100 mL         
  • Beaker, PP, 250 mL         
  • Benedict's reagent (qualitative), 30 mL          
  • Biuret reagent, 30 mL           
  • Bottle, PP, 125 mL (Fertilizer A is shipped in this)         
  • Bromothymol blue, 15 mL        
  • Centrifuge tubes, 15 mL, w/cap (6)         
  • Centrifuge tubes, 50 mL, w/cap (6)         
  •  Chromatography paper, 8.5"x11" sheet          
  • BC03 Culture, mixed bacteria3 (optional, see below)          
  • Cylinder, graduated, PP, 10 mL         
  • Cylinder, graduated, PP, 100 mL          
  •  Dextrose (d-glucose), 20 g          
  • Fertilizer, concentrate A, 125 mL (to make 12.5 liters of nitrogen-free fertilizer)          
  • Fertilizer, concentrate B, 30 mL (to make 12.5 liters of nitrogen-free fertilizer)           
  • Fertilizer, concentrate C, 15 mL (to make 12.5 liters of nitrogen-free fertilizer)          
  • Forceps          
  • Funnel          
  • Gelatin powder           
  • l-Glutamine           
  • Glycerol, 15 mL          
  • Goggles, chemical splash (w/ cap vents)        
  • Gum arabic powder          
  • Hydrochloric acid, 6 M, 30 mL         
  • Inoculating loop         
  • Lead(II) acetate, 0.1 M, 5 mL         
  • Magnifier          
  • Methyl Cellulose, 15 mL          
  • Petri dishes*         
  • Pipettes, PE          
  • Prepared slides set (optional, see below)          
  • Reaction plate, 24-well         
  • Reaction plate, 96-well         
  • Resorcinol, 0.25% (Seliwanoff's reagent A), 15 mL         
  • Rhizobia culture3, 4         
  • Ruler, 6”/150mm         
  • Scalpel        
  • Seeds: lima bean, carrot3         
  • Slides, flat, frosted, 1”x3”, pack of 72          
  • Slides, thick cavity, 1”x3” x 3mm thick, pack of 6          
  • Slide cover slips, pack          
  • Spatula, stainless, 4”, flat/spoon          
  • Sodium borate, 0.1% w/r to boron, 30 mL           
  • Sodium dodecyl sulfate, 10% w/w, 15 mL           
  • Sodium hydroxide, 6 M, 30 mL           
  • Stain: Eosin Y, 15 mL          
  • Stain: Hucker's Crystal Violet, 15 mL         
  • Stain: Iodine Gram's, 15 ml          
  • Stain: Methylene Blue, 15 mL           
  • Stain: Safranin O, 15 mL           
  • Stain: Sudan III, 15 mL          
  • Stirring rod, 6” x 5mm          
  • Stoppers          
  • Teasing needle, bent          
  • Teasing needle, straight          
  • Test paper, wide-range pH           
  • Test paper, phenylthiocarbamide (PTC)          
  • Test tubes, 16x100mm (6) stored in 50mL centrifuge tubes        
  • Test tube brush          
  • Test tube clamp          
  • Test tube rack, polypropylene, autoclavable         
  • Thermometer         
  • Turmeric reagent, 15 mL           
  • Yeast3
  • Supplemental DVD (see below) 
  •    

     * BK01 kits originally included a 10-pack of pre-sterilized polystyrene (plastic) Petri dishes. Later BK01 kits substituted a 3-pack of glass Petri dishes for the plastic Petri dishes. Because of breakage problems (both during shipping and during use) current BK01A kits include a 6-pack of 50mm polypropylene (PP) Petri dishes. The PP dishes can be re-used indefinitely simply by washing them and sterilizing them in a pressure cooker (15 minutes at 15 PSI) after filling them with agar gel. Also, because they are smaller diameter (50mm versus 75mm), the PP Petri dishes use expensive agar more efficiently. A 25 mL batch of agar medium is sufficient to fill the six Petri dishes to the proper level.      

Notes:

     1 included only in kits shipped to Hawaii (to substitute for the Rhizobia culture)      2 the book lists the BK01 kit as including amoxicillin capsules. Current BK01A kits substitute a vial of penicillin G potassium for the amoxicillin. You can substitute one heaping loose spatula spoon of penicillin G potassium powder for one 250 mg amoxicillin capsule.      3 with the exceptions of these items, the consumables in the kits should remain usable for at least two years if unopened or if carefully resealed after opening, and if stored in a cool, dark place. The Rhizobia culture, the lima bean seeds, and the yeast should remain usable for at least one year unopened. If you intend to reuse the kit for another student within two to three years, you can purchase replacements for the Rhizobia culture and the seeds at most lawn and garden stores (make sure the Rhizobia culture you purchase is compatible with lima beans), and yeast at any supermarket. Although we cannot guarantee shelf live for the BC03 Bacteria Culture because shipping and local storage conditions are beyond our control, if unopened and stored at room temperature in the dark, the BC03 culture should remain usable for at least one year and possibly several years. Do not refrigerate this culture. If you open the culture using aseptic procedures, you can recap and reseal it without significantly affecting the shelf life of the remainder.      4 not included in kits shipped to Hawaii (State regulations prohibit us from shipping Rhizobia cultures to Hawaii).