BK01 Standard/Honors Home School Biology Laboratory Kit

□ Acetic acid, 6 M, 15 mL
□ Agar powder, 10 g (sufficient to prepare 500 mL of agar medium to pour 40+ 75mm Petri dishes) 
□ Alligator clip lead, black
□ Alligator clip lead, red
□ Ammonia, 6 M, 15 mL
□ Ammonium nitrate¹
□ Antibiotic powder, penicillin²
□ Antibiotic powder, chortetracycline
□ Antibiotic powder, sulfadimethoxine
□ Antibiotic solution, neomycin
□ Ascorbic acid tablets, 500 mg
□ Barfoed's reagent, 30 mL
□ Beaker, PP, 50 mL
□ Beaker, PP, 100 mL
□ Beaker, PP, 250 mL
□ Benedict's reagent (qualitative), 30 mL
□ Biuret reagent, 30 mL
□ Bottle, PP, 125 mL (Fertilizer A shipped in this)
□ Bromothymol blue, 30 mL
□ Centrifuge tubes, 15 mL, w/cap (6)
□ Centrifuge tubes, 50 mL, w/cap (6)
□ Chromatography paper, 8.5x11" sheet
□ Culture, mixed bacteria³ (optional, see below)
□ Cylinder, graduated, PP, 10 mL
□ Cylinder, graduated, PP, 100 mL
□ Dextrose (d-glucose), 20 g
□ Fertilizer, concentrate A, 125 mL (sufficient for 12.5 liters of working strength nitrogen-free fertilizer)
□ Fertilizer, concentrate B, 30 mL (sufficient for 12.5 liters of working strength nitrogen-free fertilizer)
□ Fertilizer, concentrate C, 15 mL (sufficient for 12.5 liters of working strength nitrogen-free fertilizer)
□ Forceps
□ Funnel
□ Gelatin powder
□ l-Glutamine
□ Glycerol, 15 mL
□ Goggles, chemical splash (w/ cap vents)
□ Gum arabic powder
□ Hydrochloric acid, 6 M, 30 mL
□ Inoculating loop
□ Lead(II) acetate, 0.1 M, 15 mL
□ Magnifier
□ Methyl Cellulose, 15 mL
□ Petri dishes^*
□ Pipettes, PE
□ Prepared slides set (optional, see below)
□ Reaction plate, 24-well
□ Reaction plate, 96-well
□ Resorcinol, 0.25% (Seliwanoff's reagent A), 15 mL
□ Rhizobia culture^{3, 4}
□ Ruler, 6”/150mm
□ Scalpel
□ Seeds, carrot³
□ Seeds, lima bean³
□ Slides, flat, frosted, 1”x3”, pack of 72
□ Slides, deep cavity, 1”x3” x 3mm thick, pack of 6
□ Slide cover slips, one ounce
□ Spatula, stainless, 4”, flat/spoon
□ Sodium borate, 0.1% w/r to boron, 30 mL
□ Sodium dodecyl sulfate, 10% w/w, 15 mL
□ Sodium hydroxide, 6 M, 30 mL
□ Stain: Eosin Y, 15 mL
□ Stain: Gram's Iodine⁵
□ Stain: Hucker's Crystal Violet, 15 mL
□ Stain: Methylene Blue, 15 mL
□ Stain: Safranin O, 15 mL
□ Stain: Sudan III, 15 mL
□ Stirring rod, 6” x 5mm
□ Stoppers
□ Teasing needle, bent
□ Teasing needle, straight
□ Test strips, wide-range pH (vial)
□ Test strips, phenylthiocarbamide (PTC) (vial)
□ Test tubes, 16x100mm (6)
□ Test tube brush (1)
□ Test tube clamp (1)
□ Test tube rack, polypropylene
□ Thermometer
□ Turmeric reagent, 15 mL
□ Yeast³

□ Supplemental DVD (see below)

^* BK01 kits formerly included a 10-pack of pre-sterilized polystyrene (plastic) Petri dishes. Current BK01 kits substitute a 3-pack of 75x82x15mm glass Petri dishes for the plastic Petri dishes. Plastic Petri dishes must be discarded after one use because they cannot be re-sterilized. Glass Petri dishes can be re-used indefinitely simply by washing them and sterilizing them in a pressure cooker (15 minutes at 15 PSI) or a standard kitchen oven (30 minutes at 450F).

¹ included only in kits shipped to Hawaii (to substitute for the Rhizobia culture)
² the book lists the BK01 kit as including amoxicillin capsules. Current BK01 kits substitute a vial of penicillin G potassium for the amoxicillin. You can substitute one heaping loose spatula spoon of penicillin G potassium powder for one 250 mg amoxicillin capsule.
³ with the exceptions of these items, the consumables in the kits should remain usable for at least two years if unopened or if carefully resealed after opening, and if stored in a cool, dark place. The Rhizobia culture, the carrot and lima bean seeds, and the yeast should remain usable for at least one year unopened. If you intend to reuse the kit for another student within two to three years, you can purchase replacements for the Rhizobia culture and the seeds at most lawn and garden stores (make sure the Rhizobia culture you purchase is compatible with lima beans), and yeast at any supermarket. Although we cannot guarantee shelf live for the BC03 Bacteria Culture because shipping and local storage conditions are beyond our control, if unopened and stored at room temperature in the dark, the BC03 culture should remain usable for at least one year and possibly several years. Do not refrigerate this culture. If you open the culture using aseptic procedures, you can recap and reseal it without significantly affecting the shelf life of the remainder.
⁴ not included in kits shipped to Hawaii (State regulations prohibit us from shipping Rhizobia cultures to Hawaii)
⁵ the book lists the BK01 kit as containing 15 mL of Gram's iodine stain. Current BK01 kits substitute a 30 mL bottle of iodine-iodide solution, a more concentrated form of Gram's iodine. For Gram staining, simply dilute one part of the iodine-iodide solution with two parts distilled water, e.g., 1 drop of iodine-iodide solution mixed with two drops of water.

Please note that many of the materials included in the BK01 kits are hazardous if handled or used improperly. Before opening or using the kit, please take the time to read the Material Safety Data Sheets, and observe all recommended precautions and safety measures while using the kit.